world of amoeboid organisms

Menu

Cultures

In order to study the behavior of amoebae it may be useful to culture them. When you don’t have a sophisticated laboratory at hand, this can be done simply by supplying the amoebae with water from their natural habitat. Another simple “pond” method, which I use successfully for larger amoebae, is following. I fill petri dishes with water from the jar in which I find amoebae. In each petridish I drop a boiled maize grain. When I find an amoeba with my microscope, I carefully remove the cover slip and wash the slide in the petridish. When everything develops well, you can see several or many amoebae within some weeks.  After some time, you need to make new cultures, because the old ones get polluted. Transfer some amoeba from the old dishes to the new ones. This subculturing can be done ones a month.
In this simple manner I had stable cultures for many years. Be aware of predators and remove them. This “pond” method is very useful in that amoebae can be kept constantly available with a minimum of equipment and attention.

Recent posts

Haplomyxa saranae

Haplomyxa saranae Dellinger, 2014 Diagnosis: cell body from small rounded (0.1 mm) to large elongated shape (up to 3.5 x 0.3 mm), usually flat but

Read More »

Haplomyxa spec.

Main cell body Haplomyxa spec. Description: The cell has a central body from which numerous granuloreticulopodia emerge. The cell body can be small or large,

Read More »

Haplomyxa spec.

Haplomyxa spec. These video’s show an undescribed Haplomyxa species which was collected from a mesotrophic freshwater pond in the Netherlands, January 2020, and kept in Petri

Read More »

Chlamydophrys-schaudinn

Fritz Schaudinn (1871 – 1906) was a German zoologist. He was the co-discoverer of the causative agent of syphilis and did research on amoebas, particularly

Read More »

Alabasta longicollis

Alabasta longicollis, after Penard, 1890 Alabasta longicollis  (Penard, 1890) Diagnosis: Test pyriform, very elongated (3 times as long as wide), transparent, very little compressed, embedded

Read More »

Alabasta kivuense

Alabasta kivuense, after Gauthier-Lièvre and Thomas, 1961 Alabasta kivuense  Gauthier-Lièvre and Thomas, 1961 Diagnosis: In frontal view, the test is finger-shaped with subparallel sides, the

Read More »