Microworld

world of amoeboid organisms

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Equipment

My workhorse, the Olympus BX51

 

Microscopes

I investigate my samples using an Olympus BX51 microscope, with Nomarsky DIC, phase contrast and fluorescense, equipped with several UPlanFl and UPlanApo objectives, and a Leitz Orthoplan microscope, equipped with several plan apochromatic objectives, standard Köhler illumination, phase contrast and Smith T DIC system.
The observed ma­terial is transferred to standard microscope slides and covered with a 24 x 32 cover slip.
I use very thin modified glass pipettes for isolating specimens. A glass tube is heated over a flame in the middle and then when the glass is soft I pull both ends slowly to create a very thin pipette. I use this directly to collect amoebae. Much patience is needed and usually some level of cursing before you can be successful…
I use a Nikon Diaphot invert microscope A Nikon Diaphot inverted microscope equipped with Phase Contrast optics was used for searching through samples and for isolating specimens. quick searching samples, for isolating amoebae and for looking through cultures. After isolating the shell, I cover it with a slip, supported by some pieces of a broken cover slip.

 

Photomicrography and video

I use a Nikon Diaphot inverted microscope equipped with Phase Contrast optics for searching through samples and cultures and for isolating specimens. For filming and photographing living specimens I use a Canon EOS 5D Mark II camera on the aforementioned microscope and a Touptek E3ISPM 20MP camera on the Olympus BX51. Adobe Photoshop and ToupView© software are used for image processing and measurements. Video is also captured with ToupView and reworked with Camtasia Studio.

 

Penard slide

Slide made by Eugene Penard of Euglypha brachiata, which he sent to Dr. A.A. de Groot. Collection Ferry Siemensma.
Recent posts

Metaheleopera

M. ovalistoma – after Bartos, 1963 Metaheleopera  Bartos, 1963 Diagnosis: Shell clearly narrowing towards the pseudostome, which can be seen even more clearly on the narrower sides

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Matsakision radixicola

M. radixicola – after Chardez, 1974 Matsakision radixicola  Chardez, 1974 Diagnosis: Shell ovoid, slightly compressed, transparent; pseudostome oval, truncated perpendicularly, with a slight neck. Shell

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Matsakision cassagnaui

M. cassagnaui – after Bonnet, 1967 Matsakision cassagnaui  Bonnet, 1967 Diagnosis: Shell hyaline, elongated ovoid, very little compressed, truncated perpendicularly or very slightly obliquely in

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Matsakision

Matsakision – after Bonnet, 1967 Matsakision   Bonnet, 1967 Diagnosis: Shell hyaline, elongated ovoid, very little compressed, truncated perpendicularly or very slightly obliquely in the region

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Arcella marginata

Arcella marginata, after Deflandre, 1929 Arcella marginata Daday, 1905 Diagnosis: Shell roughly hemispherical, rather overhanging, with a flattened, smooth edge. In dorsal view showing a

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Arcella lobostoma

Arcella lobostoma, after Deflandre, 1929 Arcella lobostoma Deflandre, 1929 Diagnosis: Shell hemispherical. In ventral view, irregular circular outline, often a little elliptical; pseudostome irregularly lobed:

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