I investigate my samples using an Olympus BX51 microscope, with Nomarsky DIC, phase contrast and fluorescense, equipped with several UPlanFl and UPlanApo objectives, and a Leitz Orthoplan microscope, equipped with several plan apochromatic objectives, standard Köhler illumination, phase contrast and Smith T DIC system.
The observed material is transferred to standard microscope slides and covered with a 24 x 32 cover slip.
I use very thin modified glass pipettes for isolating specimens. A glass tube is heated over a flame in the middle and then when the glass is soft I pull both ends slowly to create a very thin pipette. I use this directly to collect amoebae. Much patience is needed and usually some level of cursing before you can be successful…
I use a Nikon Diaphot invert microscope A Nikon Diaphot inverted microscope equipped with Phase Contrast optics was used for searching through samples and for isolating specimens. quick searching samples, for isolating amoebae and for looking through cultures. After isolating the shell, I cover it with a slip, supported by some pieces of a broken cover slip.
Photomicrography and video
I use a Nikon Diaphot inverted microscope equipped with Phase Contrast optics for searching through samples and cultures and for isolating specimens. For filming and photographing living specimens I use a Canon EOS 5D Mark II camera on the aforementioned microscope and a Touptek E3ISPM 20MP camera on the Olympus BX51. Adobe Photoshop and ToupView© software are used for image processing and measurements. Video is also captured with ToupView and reworked with Camtasia Studio.