Polychaos annulatum
Polychaos fasciculatum

 

Polychaos fasciculatum (Penard, 1902)

Diagnosis: Polypodial, palmate amoeba with short, parallel pseudopodia and large fasciculate uroid, especially at the beginning of locomotion. Length in locomotion 65-140 µm (average 110 µm), monopodial, clavate locomotive forms may appear under low oxygen tension (Page, 1988). Crystals are truncate bi-pyramids. Floating form of radial type; several thick pseudopodia of unequal length radiate from irregular central mass of the granuloplasm. Single spherical nucleus, 11-23 µm in diameter, with nucleolar material in few narrow peripheral lobes, usually crescent shaped, sometimes in a single curved band, twisting inside the nucleus.

Ecology: Freshwater, Europe.

Remarks: Surface coat consists of very fine filaments, often collapsing under inappropriate fixation. This species may be confused with Polychaos annulatum, however the latter has very distinct nuclear structure. Careful examination of 3D organization of the nucleolus always excludes any doubts about the species identification. Comparison of Polychaos fasciculatum-like amoebae was done by Smirnov & Goodkov (1998).

Polychaos annulatum
Polychaos fasciculatum, with crescent shaped nucleus
Polychaos annulatum
Polychaos fasciculatum, with nucleus and bipiramid crystals
Polychaos annulatum
Polychaos annulatum
Polychaos annulatum
Polychaos annulatum nucleus
Notice the small central granule in both nuclei, see also very first image
Polychaos annulatum nucleus
Polychaos annulatum nucleus
Polychaos fasciculatum, nucleus
Polychaos annulatum
Polychaos annulatum
Polychaos fasciculatum, nucleus and drawing from Penard, 1902
Polychaos annulatum
Polychaos annulatum
Polychaos annulatum
Polychaos annulatum
Polychaos annulatum
Polychaos fasciculatum, with nucleus and bipiramid crystals

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Polychaos annulatum
Polychaos fasciculatum, with nucleus and bipiramid crystals.
Polychaos annulatum
Polychaos fasciculatum
Polychaos annulatum
Polychaos fasciculatum
Polychaos annulatum
Polychaos fasciculatum: A. Polypodium; B. Monopodium; C. Fan-shaped polypodium; D. small specimen with a Mayorella-look; F-H. Nuclei; E. Crystals. (Ferry Siemensma, 1987)
Polychaos annulatum
Polychaos fasciculatum  A-C. Branching monopodia; D. Amoeba between cover slip and microscope slide; E. Amoeba with a smooth uroid; F. Papillated uroid; G. Broad monopodium; H Small specimen, with Mayorella-look; J. Normal nucleus; I-K. Deuteramoeba spec.? (Ferry Siemensma, 1987)
Polychaos annulatum
Polychaos annulatum
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