Trichamoeba mycophaga Chakraborty & Old, 1986


Diagnosis: amoebae palmate to elongate, predominantly polytactic but may be monotactic in continuous locomotion, appearing tubular or clavate with a villous-bulb uroid. Hyaline ectoplasmic cap on pseudopodia and at advancing margin of monotactic cells. Endoplasm highly granular with elongated and bipyramidal crystals up to 2.5 µm long. Usually a single contractile vacuole, 3-15 µm in diameter, located in the posterior region of the cell. Amoebae uninucleate with spherical to oval nuclei, 4-10 µm in diameter. Nucleolar material organized in a single unfragmented structure, oval to elongate, 2.8-5.0 µm in diameter. Cysts rounded to oval, ecto- and endocyst walls separated by amorphous material several um in thickness.


Dimensions: 45-136 µm long and 25-94 µm wide. Cysts rounded to oval, 21-60 µm in diameter, nucleus 4-10 µm in diameter.


Ecology: soil. Feeding on fungal spores and bacteria.


Remarks: Amoebae in locomotion often appear palmate to elongate in shape. A slender tubular shape typical of the so-called "limax" form also frequently occurs. A distinctive floating form has not been observed. The uroid appears to be ectoplasmic and is often connected to the main body of the limax trophozoite by a narrow isthmus.